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The structural plasticity of photosystem I – light harvesting complex I (PSI-LHCI) is reflected in the recent resolution of diverse PSI-LHCI macromolecular organization states in the green alga Chlamydomonas reinhardtii: PSI-LHCI1,2, PSI-LHCI – light-harvesting complex II (LHCII)3,4 and dimeric PSI-LHCI5. It has been proposed that dynamic changes in PSI subunit composition and LHCI configuration induce macromolecular PSI-LHCI remodelling: The small transmembrane PSI subunits PSAG and PSAH together with the antenna proteins LHCA2 und LHCA9 may comprise a structurally interlinked regulatory module controlling the macromolecular organization of PSI-LHCI. Adding another level of regulation, PSI-LHCI structural remodelling might be influenced by dynamic post-translational modifications: Both PSAG and PSAH have been observed phosphorylated in C. reinhardtii in response to high light or anoxia, with PSAG phosphorylation depending on STT76,7. This study investigates the impact of a CRISPR-mediated knockout of PSAG and PSAH on the composition and macromolecular organization of PSI-LHCI in C. reinhardtii, along with its potential implications on light harvesting and electron transfer. Furthermore, this study takes advantage of chemical protein crosslinking-coupled mass spectrometry to identify potential further interaction partners of PSI-LHCI in C. reinhardtii [8].
1: Suga et al., Nature Plants, 2019
2: Su et al., Nature Plants 2019
3: Pan et al., Nature Plants, 2021
4: Huang et al., Nature Communications, 2021
5: Naschberger et al., Nature Plants, 2022
6: Bergner et al, Plant Physiology 2017
7: Younas et al., Bioscience Reports, 2022
8: Mosebach et al., Plants, 2024